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tissuelyser lt  (Qiagen)


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    Structured Review

    Qiagen tissuelyser lt
    Tissuelyser Lt, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3804 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stainless+steel+bead/Stainless+Steel+Beads/pm42869257-290-19-21
    Average 99 stars, based on 3804 article reviews
    tissuelyser lt - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Tissue:

    Article Title: Developmental contexts of gene-regulatory evolution following whole genome duplication in salmonids.
    Article Snippet: In brief, total RNA extraction from tissues of salmon was performed using a column-based miRNeasy Mini kit (Qiagen, no. 217004) following the manufacturer’s instructions. .. Each tissue was removed from RNAlater solution, placed in 700 μl Qiazol with one 5-mm stainless steel bead (Qiagen, no. 69989), homogenized with a TissueLyser (Qiagen) for 15–40 s (until the solution appeared clear), then mixed with chloroform and centrifuged at 12,000g for 15 min. .. The upper phase was extracted and mixed with 100% ethanol, passed through the RNeasy filter included in the kit, then washed, DNAse-digested (Qiagen, no. 79254) and washed again.

    Homogenization:

    Article Title: Safety, efficacy, and distal nerve Schwann cell biodistribution in mice and NHPs to support translation of AAV9 RNAi therapy for CMT1A
    Article Snippet: Briefly, genomic DNA was extracted using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Applied Biosystems, A36570 ) from mouse PNS, CNS and peripheral tissues (i.e., lumbar roots, sciatic nerve, femoral nerve, brain, liver, kidney, lung, heart, and quadriceps). .. Briefly, tissues were lysed using a 5-mm stainless steel bead (Qiagen: 69989) for all nerves or 1.0-mm Zirconia Beads (BioSpec Products: 11079110ZX) for all other tissues, followed by homogenization cycles on a TissueLyser II (Qiagen). .. Following droplet generation on a Bio-Rad QX200 AutoDG ddPCR system (Bio-Rad, France), the emulsion was transferred to a PCR plate and cycled using the following thermal cycler conditions: pre-denaturation at 95°C for 5 min, 40 cycles at 95°C for 30 s, 60°C for 1 min with ramp-rate set at 50%, and a final step at 60°C for 10 min. Data acquisition and analysis were performed on a QX200 Droplet Reader and QuantaSoft Software (Bio-Rad).



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